Effect of physicochemical anomalies of soda-lime silicate slides on biomolecule immobilization.

نویسندگان

  • Stella H North
  • Evgeniya H Lock
  • Tiffany R King
  • James B Franek
  • Scott G Walton
  • Chris R Taitt
چکیده

Glass microscope slides are considered by many as the substrate of choice for microarray manufacturing due to their amenability to various surface chemistry modifications. The use of silanes to attach various functional groups onto glass slides has provided a versatile tool for the covalent immobilization of many diverse biomolecules of interest. We recently noted a dramatic reduction in biomolecule immobilization efficiency on standard microscope slides prepared using a well-characterized silanization method. A survey of commercial soda-lime slides yielded the surprising result that slides purchased prior to 2008 had superior immobilization efficiencies when compared to those purchased after 2008. Characterization of the slides by X-ray photoelectron spectroscopy (XPS), contact angle measurements, and atomic force microscopy (AFM), revealed a significant correlation (R > 0.9) between magnesium content, surface roughness, and bioimmobilization efficiency. High performance slides had higher magnesium content and higher root-mean-square (rms) roughness (P < 0.005) than slides with lower bioimmobilization efficiencies. Although the exact mechanism of how magnesium content and surface roughness affect silane deposition has not yet been defined, we show that recent changes in the chemical and physical properties of commercial soda-lime slides affect the ability of these slides to be covalently modified.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

X-ray-assisted formation of gold nanoparticles in soda lime silicate glass: suppressed Ostwald ripening.

The in situ formation of gold nanoparticles in soda lime silicate glass under constant x-ray irradiation is compared with the ex situ formation in preirradiated glasses. The ASAXS measurements confirm that pure Au particles are formed. The comparison shows that the number of particles nucleated under irradiation is about an order of magnitude higher than of those nucleated with preirradiation. ...

متن کامل

Effect of KNO3 Molten Bath Na Enrichment on the Mechanical Performances of Ion-exchanged Soda-Lime- Silicate Glass

Systematic doping of pure molten KNO3 bath by sodium nitride was used to analyze the effect of salt poisoning on the mechanical performances of chemically tempered soda-lime-silicate float glass. The ion-exchange process appears partially influenced by the presence of limited amount of Na in the potassium nitride bath. The interdif‐ fusion coefficient and the penetration depth of K are invarian...

متن کامل

Effect of ZnO on the Physical Properties and Optical Band Gap of Soda Lime Silicate Glass

This manuscript reports on the physical properties and optical band gap of five samples of soda lime silicate (SLS) glass combined with zinc oxide (ZnO) that were prepared by a melting and quenching process. To understand the role of ZnO in this glass structure, the density, molar volume and optical band gaps were investigated. The density and absorption spectra in the Ultra-Violet-Visible (UV-...

متن کامل

Trends in Effective Diffusion Coefficients for Ion-Exchange Strengthening of Soda-Lime-Silicate Glasses

Monovalent cations enable efficient ion-exchange processes due to their high mobility in silicate glasses. Numerous properties can be modified in this way, e.g., mechanical, optical, electrical, or chemical performance. In particular, alkali cation exchange has received significant attention, primarily with respect to introducing compressive stress into the surface region of a glass, which incr...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Analytical chemistry

دوره 82 1  شماره 

صفحات  -

تاریخ انتشار 2010